Fig. 1: Intramyocardial injection of hiPSC-ECs improves the formation of de novo vessels and cardiac function in MI-induced hearts. | Experimental & Molecular Medicine

Fig. 1: Intramyocardial injection of hiPSC-ECs improves the formation of de novo vessels and cardiac function in MI-induced hearts.

From: Enhancement strategy for effective vascular regeneration following myocardial infarction through a dual stem cell approach

Fig. 1

a Representative images of blood vessels stained with IB4-rhodamine (red) in the infarct zone, border zone, and remote zone and at 8 weeks after injection of hiPSC-ECs and their quantification summary. For quantification, the number of capillaries in five randomly selected fields (mm2) in each heart was counted. n = 5. *p < 0.05. Scale bars: 100 µm. b Representative image of blood vessels newly formed by iPSC-ECs-GFP (green), IB4-rhodamine (red) and DAPI (blue). Scale bars: 20 µm. c–j Rats undergoing MI were intramyocardially injected with hiPSC-ECs or control cells, followed by echocardiography analysis. c The schematic timeline from MI modeling and transplantation of iPSC-EC to measurement of cardiac function. d Left ventricular ejection fraction (EF), (e) left fractional shortening (FS), (f) left ventricular internal diastolic dimension (LVIDd), (g) left ventricular internal systolic dimension (LVIDs), (h) septal wall thickness (SWT), (i) posterior wall thickness (PWT), and (j) relative wall thickness (RWT). n = 6. *p < 0.05. k Representative images showing cardiac fibrosis after staining with Masson’s trichrome in the hearts harvested 8 weeks after cell treatment. Quantification results of cardiac fibrosis (l) and viable myocardium (m). n = 5. *p < 0.05. Scale bars: 2000 µm.

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