Fig. 1: Characterization of PBMSCs. | Experimental & Molecular Medicine

Fig. 1: Characterization of PBMSCs.

From: β-Catenin promotes long-term survival and angiogenesis of peripheral blood mesenchymal stem cells via the Oct4 signaling pathway

Fig. 1

a Representative images of primary cultures of rat PBMSCs from normal rats at Day 14 and traumatized rats at Day 14 and Day 21 and the fourth passage of PBMSCs. Scale bars = 200 µm. b Flow cytometry analysis of PBMSC-positive surface markers for the MSC markers CD44, CD71, CD90, and CD105 and negative markers for the HSC markers CD34 and CD45 and EPC markers CD31 and CD133. c–g PBMSCs were induced to differentiate into adipocytes, chondrocytes, osteoblasts, and vascular cells. c Adipogenesis-committed differentiation of PBMSCs stained by oil red O (red). d Chondrogenesis-committed differentiation of PBMSCs stained with alcian blue (blue). e Osteogenesis-committed differentiation of PBMSCs stained by alizarin red (red). f Staining after normal culture with a dye-free solution. Scale bars = 100 µm. g Vascular endothelial cells were examined via immunofluorescence for the expression of the ectodermal cell markers Factor VIII (red) and α-SMA (green). DAPI staining (nuclei; blue) and merged images are also shown. Scale bars = 20 µm.

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