Fig. 2: β-catenin promotes PBMSC vitality and long-term survival. | Experimental & Molecular Medicine

Fig. 2: β-catenin promotes PBMSC vitality and long-term survival.

From: β-Catenin promotes long-term survival and angiogenesis of peripheral blood mesenchymal stem cells via the Oct4 signaling pathway

Fig. 2

The experiments were divided into the following groups: transfection of vehicle (-), lentivirus vector encoding β-catenin (oeβ-catenin), β-catenin shRNA (shβ-catenin), and β-catenin or siRNA (oeβ-catenin plus shβ-catenin). a The ability of all groups of PBMSCs to form clones was tested by cell colony formation assays and crystal violet staining. b Quantitative analysis of the number of cell clones in (a). c The growth curve of all groups of PBMSCs at 1, 2, 3, 4, 5, 6, and 7 d. d The viability of PBMSCs after 70 days of culture was tested by the MTS method. e β-Galactosidase staining (black arrowheads) was used to investigate cellular senescence in all groups of PBMSCs. Scale bars = 100 µm. f Quantitative analysis of the ratio of senescent PBMSCs to total PBMSCs in (e). g The apoptosis level of all groups of PBMSCs was assessed by flow cytometry using Annexin V/propidium iodide (PI) staining. All graphical data show the means ± SEMs. P < 0.05: * vs. the vehicle group, # vs. the oeβ-catenin group, Δ vs. the shβ-catenin group, n = 10 in each group.

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