Fig. 4: PLD1 inhibition reduces Wnt/β-catenin signaling and suppresses migration, invasion and self-renewal capacity. | Experimental & Molecular Medicine

Fig. 4: PLD1 inhibition reduces Wnt/β-catenin signaling and suppresses migration, invasion and self-renewal capacity.

From: Inhibition of phospholipase D1 induces immunogenic cell death and potentiates cancer immunotherapy in colorectal cancer

Fig. 4

a HCT 116 cells were treated with A3373 in a time- (for 36 h) and dose-dependent (at 2 μM) manner in the presence of 1% serum. The lysates were analyzed by immunoblotting. b CRC cells were treated with A3373 (2 μM) for 12 h, and the lysates were immunoprecipitated and/or immunoblotted with the indicated antibodies. c Effect of A3373 on Wnt3a (150 ng/mL)-induced TCF transactivation in HEK293 cells. d Effect of A3373 on β-catenin-mediated TCF transactivation in HEK293 cells. e Effect of A3373 on the sphere-forming capacity of DLD1 cells. Limiting dilution assays were performed by diluting sphere-cultured cells. f Effect of A3373 on Wnt3a-induced migration and invasion. The results are representative of at least three independent experiments and presented as the mean ± SD. ***P < 0.001.

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