Fig. 5: PLD1 inhibition in CRC cells enhances phagocytic activities by downregulating “do not eat-me” signals and upregulating ‘eat-me’ signals. | Experimental & Molecular Medicine

Fig. 5: PLD1 inhibition in CRC cells enhances phagocytic activities by downregulating “do not eat-me” signals and upregulating ‘eat-me’ signals.

From: Inhibition of phospholipase D1 induces immunogenic cell death and potentiates cancer immunotherapy in colorectal cancer

Fig. 5

a PLD1 expression is positively correlated with the level of the indicated “do not eat-me” signals in CRC as analyzed by TCGA database. b The lysates of CRC tissues were analyzed by immune blotting using the indicated antibodies. N adjacent nontumor, T tumor. c Effect of A3373 on the levels of the “do not eat-me” signals in HCT116 cells as analyzed by flow cytometry. d Effect of A3373 on the level of calreticulin in HCT116 cells. e Effect of A3373 on the amount of secreted HMGB1 as analyzed by ELISA. f Effect of A3373 on the level of extracellular ATP. g Effect of A3373 on the phagocytic activity of macrophages. Right, the population of CFSE+CD11b+ macrophages was quantified. The results are representative of at least three independent experiments and presented as the means ± SDs. ***P < 0.001.

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