Fig. 1: NRBF2 is upregulated after IR in glioblastoma.

a Scheme of the U-87 Luc orthotopic xenograft mouse model and mouse irradiation schedule. b Microarray experiments were performed using cDNA samples obtained from orthotopic xenograft GBM tumor sections treated or not treated with 2Gy × 5 radiation, and microarray results were deposited in the Gene Expression Omnibus database (GEO: GSE117126). c Hematoxylin and eosin (H&E) staining (left panel) and immunohistochemistry (IHC) of Ki67 and NRBF2 (middle panel and right panel) of coronal sections from U-87 MG orthotopic xenograft mouse control or treated with IR (2Gy × 5). Scale bars, 10 μm. d Real-time qRT‒PCR analysis of the nrbf2 mRNA levels in the GBM orthotopic xenograft mouse model. e Real-time qRT‒PCR analysis of the nrbf2 mRNA levels in GBM cell lines. f Western blot analysis of control and IR-treated GBM cells shows IR-induced expression of NRBF2. g The gene expression profiles were collected from The Cancer Genome Atlas (TCGA) database available through Gliovis (PMID: 28031383). mRNA expression of nrbf2 was measured in 10 nontumor tissues and 528 GBM tissues. h Nrbf2 gene expression profiles were collected from The Cancer Genome Atlas (TCGA, Pan-Cancer Atlas) database (https://www.cbioportal.org/). mRNA expression of nrbf2 was measured in nine different cancer types, including GBM. **p < 0.01, ****p < 0.0001 using an unpaired t test.