Fig. 2: Lnc Tmem235 inhibits hypoxia-induced apoptosis of BMSCs. | Experimental & Molecular Medicine

Fig. 2: Lnc Tmem235 inhibits hypoxia-induced apoptosis of BMSCs.

From: Lnc Tmem235 promotes repair of early steroid-induced osteonecrosis of the femoral head by inhibiting hypoxia-induced apoptosis of BMSCs

Fig. 2

a Localization of Lnc Tmem235 in the genome; b Western blot detection of myc fusion protein to evaluate the coding ability of Lnc Tmem235 (n = 3), and KLF4 was used as the coding protein control; Kruppel-like factor-4 (KLF4); c RNA-FISH detection of subcellular localization of Lnc Tmem235 (n = 5), and 18 S and U6 were used as positive controls for the cytoplasm and nucleus, respectively; RNA fluorescence in situ hybridization (RNA-FISH); d The expression of Lnc Tmem235 was detected by qPCR (n = 6); empty vector (GV367); e–h The expression levels of Bcl-2, Bax, and CASP3 were analyzed by western blotting (n = 3); i, k Apoptosis was detected by TUNEL/DAPI (n = 5); TdT-mediated dUTP nick-end labeling (TUNEL); j, l Apoptosis was detected by Annexin V/PI (n = 5). In (d, f–h, k–l), the data are normally distributed, and the variance is homogeneous. Data are presented as the means ± SDs; statistical significance was calculated by one-way ANOVA with Tukey’s post-hoc tests; *P < 0.05.

Back to article page