Fig. 2: HIF-1α and EIF4A3 regulate circRNF13 expression.
From: Hypoxia-induced circRNF13 promotes the progression and glycolysis of pancreatic cancer
a DNA-binding motifs of HIF-1α on the promoters of RNF13. b Schematic diagram of the putative binding sites of HIF-1α on the RNF13 promoter. c ChIP–qPCR experiments were conducted to determine the binding of HIF-1α to the RNF13 promoter using an anti-HIF-1α antibody. d Schematic illustration of wild-type and mutant reporter plasmids. e Relative luciferase activities in SW-1990 and MIA PaCa-2 cells transfected with luciferase reporter plasmids containing the wild-type or mutant RNF13 promoter sequence and HIF-1α overexpression plasmids. f qRT–PCR analysis of the relative expression of pre-mRNA and mRNA of RNF13 under hypoxia. g The CircInteractome database was used to predict the binding sites of EIF4A3 in the downstream and upstream regions of circRNF13 pre-mRNA. h RNA pull-down assay determined that EIF4A3 could bind to both downstream and upstream sequences. i RIP-qPCR confirmed the binding sites of EIF4A3 in the flanking sequence of circRNF13. j qRT–PCR analysis of circRNF13 expression after EIF4A3 overexpression and knockdown in PC cells. Data represent at least three independent experiments and are presented as the means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.
