Fig. 3: CircRNF13 promotes PC proliferation and angiogenesis in vitro and in vivo.
From: Hypoxia-induced circRNF13 promotes the progression and glycolysis of pancreatic cancer
a, c A colony formation assay was conducted to determine the proliferation ability of SW-1990 and MIA PaCa-2 cells after circRNF13 was overexpressed or knocked down. b, d EdU assay further confirmed the proliferation ability of SW-1990 and MIA PaCa-2 cells after circRNF13 was overexpressed or knocked down (scale bar: 100 μm, magnification: 200x). e, i Representative images of the tumor xenograft model of each group were obtained by bioluminescence imaging. f, j The line graphs show the growth of tumors as monitored every 3 days. g, k The subcutaneous tumors were removed after 21 days. h, l The weights of tumors were evaluated. m Ki-67 and CD31 expression in tumor tissues of the circRNF13-overexpressing group and control group (scale bar: 100 μm, magnification: 200x). n A tube formation assay was conducted to confirm the effect of circRNF13 on angiogenesis. Data represent at least three independent experiments and are presented as the means ± SD. *P < 0.05, **P < 0.01.
