Fig. 7: CircRNF13 modulates the miR-654-3p target PDK3.
From: Hypoxia-induced circRNF13 promotes the progression and glycolysis of pancreatic cancer
a Hierarchical clustering of significantly differentially expressed mRNAs in MIA PaCa-2 cells under normal and hypoxic conditions. b Schematic illustration showing the targets of miR-654-3p. c qRT–PCR was performed to measure gene expression under hypoxia. d PDK3 mRNA and protein levels after PC cells were transfected with miR-654-3p mimics or inhibitor. e Schematic illustration of wild-type and mutant reporter plasmids. f The luciferase activities in MIA PaCa-2 and SW-1990 cells transfected with miR-654-3p mimics or mimics NC. g The effects of circRNF13 and miR-654-3p on the expression of PDK3. h PDK3 levels in xenograft tumors removed from each group. Data represent at least three independent experiments and are presented as the means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.
