Fig. 8: CircRNF13 promotes the progression of PC via PDK3.
From: Hypoxia-induced circRNF13 promotes the progression and glycolysis of pancreatic cancer
a The knockdown efficiency of PDK3 was verified at the mRNA and protein levels in SW-1990 cells by qRT–PCR and Western blotting assays. b Knockdown of PDK3 impaired colony formation ability in SW-1990 cells. c Knockdown of PDK3 impaired cell invasion ability in SW-1990 cells (scale bar: 100 μm). d Tube formation ability of HUVECs cultured in medium collected from circRNF13-overexpressing SW-1990 cells transfected with PDK3 siRNAs or their corresponding controls. e Representative IHC images of PDK3 expression in PC tissues and corresponding normal tissues (scale bar: 200 μm, magnification: top 40x, bottom 200x). f IHC scores of PDK3 in 90 cases of PC tissues with corresponding normal tissues. g Kaplan–Meier overall survival analysis of PDK3 expression in patients with PC (N = 90, P < 0.001). h Pearson correlation analysis of the positive correlation between circRNF13 and PDK3 expression (r = 0.800, P < 0.0001). i Graphical illustration demonstrating the circRNF13/miR-654-3p/PDK3 axis in pancreatic cancer. Data represent at least three independent experiments and are presented as the means ± SD. *P < 0.05, **P < 0.01.
