Fig. 2: Conditioned medium (CM) from particulate matter (PM)-treated macrophages increases cancer cell motility and activates EGFR expression in cancer cells.
From: Particulate matter promotes cancer metastasis through increased HBEGF expression in macrophages
a Wound healing assay of A549 cells incubated with CM from PM-treated THP1 cells; images were acquired at 0 and 36 h. Scale bar, 250 μm. b Transwell migration (top) and invasion (bottom) assays of A549 cells incubated with CM from PM-treated THP1 cells. Scale bar, 200 μm. c Phospho-RTK array assay of A549 CM-Control (Con) and A549 CM-PM cells. The dots inside the rectangle represent EGFR (left). Relative pixel intensities indicating phospho-EGFR levels in the phospho-RTK array (right). d Gene set enrichment analysis plot of EGFR-induced genes in A549 CM-PM cells. NES normalized enrichment score, FWER familywise error rate, FDR false discovery rate. e Immunoblot analysis of EGFR phosphorylation in A549 cells after incubation with CM from PM-treated THP1 cells for the indicated time. β-Actin was used as the loading control. f Immunoblot analysis of EGFR phosphorylation in A549 cells after incubation with the indicated concentrations of CM from PM-treated THP1 cells at for 20 min. *P ≤ 0.05, ***P ≤ 0.001.
