Fig. 4: HBEGF secreted by particulate matter (PM)-treated macrophages increases cancer cell motility and activates EGFR in cancer cells.
From: Particulate matter promotes cancer metastasis through increased HBEGF expression in macrophages
a ELISA of conditioned medium from particulate matter-treated cells (CM-PM) immunoprecipitated with control IgG or an anti-HBEGF antibody. b Immunoblot analysis of EGFR phosphorylation in A549 cells after treatment with CM-PM immunoprecipitated with control IgG or an anti-HBEGF antibody. c Wound healing assay of A549 cells treated with CM-PM immunoprecipitated using control IgG or an anti-HBEGF antibody. Images were acquired at 0 and 36 h. Scale bar, 250 μm. d Transwell migration (top) and invasion (bottom) assays of A549 cells treated with CM-PM immunoprecipitated using control IgG or an anti-HBEGF antibody. Scale bar, 200 μm. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001; ns not significant.
