Fig. 4: Vitamin C supplementation reverses the increased body weight in SMP30-KO mice. | Experimental & Molecular Medicine

Fig. 4: Vitamin C supplementation reverses the increased body weight in SMP30-KO mice.

From: SMP30-mediated synthesis of vitamin C activates the liver PPARα/FGF21 axis to regulate thermogenesis in mice

Fig. 4

Male mice were fed an HF diet for 3 weeks (n = 7–8/group). a Serum vitamin C and b FGF21 levels were measured after overnight fasting using the fluorophore-nitroxide probe Naph-DiPy nitroxide and an ELISA kit, respectively. c Final body weight of WT, SMP30-KO, and SMP30-KO mice supplemented with vitamin C (2 g/L). d Total cholesterol, e glucose, and f triglycerides in serum were measured after overnight fasting by enzymatic colorimetric assay kits. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01 and ***P < 0.001. One-way ANOVA followed by Bonferroni post hoc tests (a–f).

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