Fig. 5: RAI14 promotes cell proliferation, migration, and invasion of TNBC. | Experimental & Molecular Medicine

Fig. 5: RAI14 promotes cell proliferation, migration, and invasion of TNBC.

From: The deubiquitinating enzyme STAMBP is a newly discovered driver of triple-negative breast cancer progression that maintains RAI14 protein stability

Fig. 5

a MDA-MB-231 and BT549 cells were transfected with two independent RAI14 siRNAs or control siRNAs. RAI14 expression in cells was measured by Western blotting. b–d Cell proliferation, migration, and invasion of MDA-MB-231 and BT549 cells transfected with two independent RAI14 siRNAs or control siRNA were determined by MTS assays (b), wound healing assays (c), and Transwell migration assays (d), respectively. Representative images are shown. The wound healing percentage and migration percentage were determined with Image-Pro Plus 7 software. e–g RNA-seq analysis in MDA-MB-231 cells following siRNA #1-mediated knockdown of RAI14. The mean ± SD (n = 3). *p < 0.05 compared to the NC. e Volcano plot of gene expression (siRAI14 versus the siControl; fold change, ≥2; p < 0.05) between RAI14 knockdown and control MDA-MB-231 cells. f Heatmap of differential gene expression between RAI14 knockdown and control MDA-MB-231 cells. g GO analysis of differentially expressed genes between RAI14 knockdown and control MDA-MB-231 cells. h MDA-MB-231 cells were transfected with two independent RAI14 siRNAs or control siRNAs. The expression of the indicated proteins in cells was measured by Western blotting.

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