Fig. 4: Comparison of bulk tissues with the sequencing results of each platform.

a Annotated colon tumor tissue (SMC-50T), hematoxylin and eosin (H&E, ×100) staining. The annotations indicated a region of stromal cells (yellow) and tumor cells (remaining cells) (upper), and the percentage of tumor cells (epithelial cells) estimated by H&E staining, bulk-RNA sequencing (ESTIMATE), snRNA-seq, and scRNA-seq (lower). b Differences in the tumor percentages between each platform and the bulk sequencing results of colon tissues (n = 4) (*, 0.01 < p ≤ 0.05; Wilcoxon signed-rank test).