Fig. 2: NAD+ stimulates mitochondrial PAR via PARP1.
From: Mitochondrial PARP1 regulates NAD+-dependent poly ADP-ribosylation of mitochondrial nucleoids
Mitochondria purified from HeLa WT (a) and PARP1-KO (b) cells were treated with the indicated combinations of reagents for 30 min at 37 °C, and the western blots were probed with the indicated antibodies. Mitochondria purified from wild-type HeLa cells were treated with the indicated concentration of H2O2 (c) or menadione (d) with/without NAD+ for 30 min at 37 °C, and the western blots were probed with antibodies as shown. e, f Purified mitochondria from HeLa WT cells were incubated with the indicated concentrations of NAD+ precursors for 30 min at 37 °C, and the western blots were probed with the indicated antibodies. Blot quantification data are shown. Two-way ANOVA was used to calculate the p value (H2O2, n = 5; menadione, n = 4; NAM/NMN and NR/NAD, n = 3). Error bars represent the standard deviation. ***, <0.001.
