Fig. 2: Spreading of synucleinopathy after intracerebral injections of the WT-syn fibril and V40G multimer α-synuclein. | Experimental & Molecular Medicine

Fig. 2: Spreading of synucleinopathy after intracerebral injections of the WT-syn fibril and V40G multimer α-synuclein.

From: Inflammation promotes synucleinopathy propagation

Fig. 2

a Representative images of mouse brain Section 10 weeks after injection of PBS, WT-syn fibrils and V40G multimers stained with phospho-α-synuclein (pS129). Scale bar, 50 μm. b Heatmap of regions affected by α-synuclein pathology at 2, 4, and 10 weeks after seed injection (asterisks indicate the injection site; n = 6 per group). c, d Western blotting of brain tissue extracts obtained 10 weeks after injection. The ratio of insoluble to soluble Tx-100 is quantified in (d). Data are expressed as the mean ± SEM, one-way ANOVA with Tukey’s post hoc test, two-sided, *P < 0.05. e Co-immunofluorescence images. Thioflavin-positive pS129 aggregates are indicated with arrows. t = 10 weeks. Scale bar, 100 μm. f Immunoelectron microscopy with a pS129 antibody. pS129-positive aggregates with granular and filamentous structures are indicated with arrows. t = 10 weeks. Scale bar, 0.5 μm.

Back to article page