Fig. 1: The survival advantage of culture-adapted hESCs under mitotic stress. | Experimental & Molecular Medicine

Fig. 1: The survival advantage of culture-adapted hESCs under mitotic stress.

From: TPX2 prompts mitotic survival via the induction of BCL2L1 through YAP1 protein stabilization in human embryonic stem cells

Fig. 1

A After treatment with YM155 (50 nM) for 24 h, the death of P1 and P4 hESCs was determined by Annexin V/7-AAD staining (n = 6 independent experiments; mean ± SEM, two-way ANOVA, ***p < 0.001). B Relative mRNA expression level of BCL2L1 in P1 and P4 hESCs (n = 5 independent biological repeats; independent experiments; mean ± SEM, Mann‒Whitney test, **p < 0.01). C, D Death of P1 and P4 hESCs after treatment with 50 ng/ml nocodazole (Noc: C) or 50 ng/ml paclitaxel (Tax: D) measured by flow cytometry and stained with Annexin V and 7-AAD (n = 5 independent experiments; mean ± SEM, two-way ANOVA, ****p < 0.0001 for C; n = 6 independent experiments, two-way ANOVA, ***p < 0.001 for D).

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