Fig. 2: BCL2L1 induces survival associated with TPX2.

A Graphical representation of chromosome 20 and the 20q11.21 locus with the accompanying genes (left) and relative expression levels of the genes at the 20q11.21 locus in P1, P2, P3, and P4 hESCs (right). B Relative transcript levels of BCL2L1 and TPX2 in P1, P2, P3, and P4 hESCs. C Immunoblotting for TPX2 and BCL-xL from the indicated hESCs; α-tubulin was utilized for equal protein loading. D The relative mRNA expression level of the indicated gene in P1 and P4 hESCs (n = 7 independent biological repeats; mean ± SEM, Mann‒Whitney test, **p < 0.01, *p < 0.05). E Relative mRNA expression levels of TPX2 and BCL2L1 in P4 hESCs 48 h after siRNA administration in the control (NC) and TPX2 groups (siTPX2#3) (n = 5 independent experiments; mean ± SEM, two-way ANOVA, ****p < 0.0001, *p < 0.05). F Relative mRNA expression levels of TPX2 and BCL2L1 in H9-iTPX2-hESCs 24 h after treatment with 0.1 μg/ml Dox (n = 4 independent experiments, mean ± SEM, two-way ANOVA, ****p < 0.0001, *p < 0.05). G Relative mRNA expression levels of TPX2 and BCL2L1 in iTPX2-hESCs at the indicated times after treatment with 0.1 μg/ml Dox. H Immunoblotting of TPX2 and BCL-xL in iTPX2-hESCs at the indicated time after treatment with 0.1 μg/ml Dox (arrow for induced GFP-TPX2, * for endogenous TPX2). Relative band densities of BCL-xL are shown. I Immunoblotting of TPX2 and BCL-xL in iTPX2-hESCs 24 h after treatment with the indicated dose of Dox. Relative band densities of BCL-xL are shown. J Copy number output using the Illumina Asian Screening Array (~700 K) computed by GenomeStudio software provided by Illumina. The two plots shown are for B allele frequency and log R ratio. The copy number gain on chromosome 20 detected in H9-P4 hESCs based on the log R ratio is shown by a red bar.