Fig. 3: TPX2 induction rescues mitotic cell death in normal hESCs.

A Graphical representation of the effect of TPX2 induction on cell death after Noc treatment in hESC-iTPX2 (left) and flow cytometry analysis of PE-Annexin V staining after iTPX2 hESCs were pretreated with 0.1 μg/ml Dox or DMSO (Mock) for 16 h in the presence or absence of nocodazole (50 ng/ml) (Noc Tx). The bar graph shows the death (%) of GFP-negative and GFP-positive cells (right) (n = 4 independent experiments; mean ± SEM, Mann‒Whitney test, *p < 0.05). B Graph of % of cell death determined by 7-AAD staining of the GFP-negative or GFP-positive population after administration of the indicated dose of Noc. C Graphical presentation of the competition assay with coculture of the indicated cell types. D, E Flow cytometry analysis of P1 and P4 hESCs (D, n = 6 independent experiments; mean ± SEM, Two-Way ANOVA, ****p < 0.0001) or P1 and iTPX2 hESCs (E, n = 5, two-way ANOVA, p value < 0.0001) after treatment with 50 ng/ml of Nocodazole for 24 h. Populations were identified by GFP after 2 days of Noc treatment. F Flow cytometry analysis of 7-AAD/Annexin V staining of P4 hESCs after control (siNC) or TPX2 siRNA (siTPX2) treatment (n = 6 independent experiments; mean ± SEM, two-way ANOVA, ****p < 0.0001).