Fig. 4: YAP/TEAD4 leads to BCL2L1 expression.

A GSEA of WP_PATHWAYS_REGULATING_HIPPO_SIGANLING (left) and GOBP_REGULATION_OF_HIPPO_SIGNALING (right) of P1/P2 hESCs and P3/P4 hESCs from the RNA-seq data of P1, P2, P3 and P4 hESCs (GSE167495). B 8X GTIIC reporter assay of P1 and P4 hESCs (n = 4 independent experiments; mean ± SEM, Mann–Whitney test, *p < 0.05). C Relative mRNA levels of YAP1, CTGF and BCL2L1 in P1 or P4 hESCs (n = 6 independent experiments; mean ± SEM, two-way ANOVA, **p < 0.01, ***p < 0.001). D Immunoblotting of TPX2, YAP1, TEAD4 and phosphorylation of YAP1 (at serine 127, S127: α-tubulin or β-actin were utilized for equal protein loading. E Immunoblotting for active YAP1 in P1 and P4 hESCs. Vinculin was used as a loading control. F Immunoblotting for YAP1 and TEAD4 in the cytosol or nuclei of P1 or P4 hESCs., α-Tubulin was used for equal protein loading. G–I Relative mRNA expression of the indicated genes in P4 hESCs after control (NC) or siRNA for (G) YAP1 (n = 3 independent experiments; mean ± SEM, two-way ANOVA, ****p < 0.0001) (H) TAZ (n = 4 independent experiments; mean ± SEM, two-way ANOVA, ****p < 0.0001), or (I) TEAD4 (n = 5 independent experiments; mean ± SEM, two-way ANOVA, ***p < 0.001, ****p < 0.0001).