Fig. 1: Hepatic catecholamine and ADRB2 expression levels are increased in EtOH-fed mice. | Experimental & Molecular Medicine

Fig. 1: Hepatic catecholamine and ADRB2 expression levels are increased in EtOH-fed mice.

From: Catecholamine induces Kupffer cell apoptosis via growth differentiation factor 15 in alcohol-associated liver disease

Fig. 1

a Serum ALT and AST levels were measured in WT mice (n = 9 for Pair, n = 11 for EtOH). Epinephrine and norepinephrine levels were measured in cecum lysates (b), portal blood (c), and liver tissue lysates (d) of WT mice (n = 9 for Pair, n = 11 for EtOH). e Correlations between serum ALT levels and cecal or hepatic catecholamine levels were assessed in EtOH-fed WT mice (n = 11). f Relative mRNA levels of adrenergic receptors and MAO enzymes in HEPs were analyzed by zonation. g Heatmap indicating the relative mRNA levels of adrenergic receptors and MAO enzymes in Pair- or EtOH-fed mouse liver tissues. h Immunoblotting of ADRB2, MAOA, and MAOB in whole liver tissues of Pair- or EtOH-fed mice (3 blots per group). i Representative immunostaining of ADRB2, MAOA, and MAOB in liver sections. Scale bars, 50 µm. Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t test. Central vein (CV) and portal triad (PT).

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