Fig. 4: scRNA-seq analysis identifies apoptotic KCs with increased Adrb2 expression after chronic EtOH intake. | Experimental & Molecular Medicine

Fig. 4: scRNA-seq analysis identifies apoptotic KCs with increased Adrb2 expression after chronic EtOH intake.

From: Catecholamine induces Kupffer cell apoptosis via growth differentiation factor 15 in alcohol-associated liver disease

Fig. 4

a–e scRNA-seq analysis of KCs isolated from Pair- or EtOH-fed mice. a Schematic diagram for the scRNA-seq of KCs with UMAP clustering plots annotating conditions (left) or clusters (right). b Feature plots for the mitochondrial gene frequencies and gene counts per cell. c Feature plots indicating the relative mRNA levels encoding KC markers (Clec4f, Vsig4, and Timd4), infiltrated macrophage markers (Ccr2 and Cx3cr1), ADRB (Adrb1 and Adrb2), and PKA subunits (Prkar1a and Prkacb). d Gene levels related to the indicated pathways were analyzed by clusters and presented by violin plots. e Selected top 50 enriched pathways in Cluster 1 vs. Cluster 3. f Annexin V assay of isolated KCs from Pair- or EtOH-fed mice (left) (n = 4/group; 3 replicates) and representative CLEC4F immunostaining of liver sections (right). Data are presented as the mean ± SEM. *p < 0.05 by Student’s t test. Scale bars, 50 µm. The illustration in Fig. 4a was created with BioRender.com.

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