Fig. 5: In situ activation of ADRB2 stimulates apoptosis of KCs in the perivenous area in a PKA-dependent manner. | Experimental & Molecular Medicine

Fig. 5: In situ activation of ADRB2 stimulates apoptosis of KCs in the perivenous area in a PKA-dependent manner.

From: Catecholamine induces Kupffer cell apoptosis via growth differentiation factor 15 in alcohol-associated liver disease

Fig. 5

a–k In situ closed liver perfusion was performed with media containing vehicle (Veh; saline) or 100 ng ml−1 LPS, 1 μM CBL, and 50 mM EtOH (LCE) with or without KT5720 (PKA inhibitor, 1 μM) (n = 3/group; 3 replicates). a Schematic diagram for the in situ closed liver perfusion. b, c Representative flow cytometry panels and Annexin V assay of F4/80hiCD11b+ KCs. d, e Representative CLEC4F immunostaining of liver sections. Perivenous area (white dashed circles) and CLEC4F+ KCs (yellow triangles). Scale bars, 50 µm. f, g Representative immunoblots for ADRB2, PKA-Cα, GDF15, and mitochondrial CYP2E1 (2 blots per group). h, i qRT‒PCR analyses of isolated KCs for genes related to ADRB/PKA signaling pathways and apoptosis (n = 3/group). Not detected (n.d.). j, k qRT‒PCR analyses of whole liver tissues (j) or isolated KCs (k) (n = 3/group). Data are presented as the mean ± SEM. *,#p < 0.05, **,##p < 0.01 by Student’s t test or by one-way ANOVA.

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