Fig. 1: Effects of Parkin knockout on the 4-week CCl4 injection-induced serum and histological changes in mice. | Experimental & Molecular Medicine

Fig. 1: Effects of Parkin knockout on the 4-week CCl4 injection-induced serum and histological changes in mice.

From: Site-specific ubiquitination of VDAC1 restricts its oligomerization and mitochondrial DNA release in liver fibrosis

Fig. 1

Blood and liver samples were collected 3 days after the final CCl4 injection. (a) Survival rate (n = 31 mice/group). (b) Quantified relative mRNA levels of Col1a1, Acta22, and Mmp2 normalized to the Gapdh levels in mouse livers (n = 5-6/group). (c) Representative images of Masson trichrome and Sirius red staining of the liver. (d) Quantified fibrotic area based on Masson trichrome staining (n = 6/group) and Sirius red staining (n = 8-9/group). (e) Serum ALT and AST levels (n = 7/group). (f) Representative TEM images of livers (n = 4-5/group). The white arrows denote damaged mitochondria (shrunk mitochondria, disorganized mitochondrial cristae, and broken mitochondrial membrane). (g) NAD+ level depicting mPTP opening. (h) Mitochondrial aconitase activity. Mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001 and **** p < 0.0001 between the indicated groups.

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