Fig. 2: Effects of Parkin ablation on autophagy and apoptosis protein markers in livers following CCl4 challenge. | Experimental & Molecular Medicine

Fig. 2: Effects of Parkin ablation on autophagy and apoptosis protein markers in livers following CCl4 challenge.

From: Site-specific ubiquitination of VDAC1 restricts its oligomerization and mitochondrial DNA release in liver fibrosis

Fig. 2

Representative immunoblots and quantitative histogram of the autophagy markers (a) Parkin, (b) Atg5, (c) Atg7, (d) p62, and (e) LC3BII/I and the cell death-related proteins (h) Bax, (i) Bcl2, (j) Caspase3, (k) cytosolic Cytochrome C, and (l) mitochondrial Cytochrome C in the liver tissues from WT and Parkin-/- mice injected with CCl4 or oil for 4 weeks. GAPDH, α-tubulin or COX IV was used as the loading control (n = 6-9/group). (f) Representative DAPI/TUNEL staining images of mouse livers. (g) Quantified TUNEL-positive nuclei (n = 4-5/group). Mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001 and **** p < 0.0001 between the indicated groups.

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