Fig. 4: Effects of Parkin knockout on mtDNA release and activation of STING signaling. | Experimental & Molecular Medicine

Fig. 4: Effects of Parkin knockout on mtDNA release and activation of STING signaling.

From: Site-specific ubiquitination of VDAC1 restricts its oligomerization and mitochondrial DNA release in liver fibrosis

Fig. 4

a Representative immunoblots of cGAS, p-STING/STING, and p-IRF3/IRF3 in WT and Parkin-/- mouse livers 4 weeks after injection of CCl4 or oil (tissues were collected 3 days after the final injection). b Relative ratio of cytosolic to total mtDNA (mt-Nd1) in mouse livers (n = 5/group). Quantitative histogram of (c) cGAS, (d) p-STING/STING, and (e) p-IRF3/IRF3 expression levels in the mouse liver. Representative immunoblots and quantitative histogram of (f) p-NFκB p65/NFκB p65, (g) IFN-β, (h) TNF-α, and (i) IL-6 (GAPDH or α-Tubulin used as the loading control) (n = 5-9/group). Mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001 and **** p < 0.0001 between the indicated groups.

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