Fig. 6: Effects of Parkin-mediated ubiquitination on VDAC1 oligomerization and cytosolic mtDNA accumulation in HepG2 cells. | Experimental & Molecular Medicine

Fig. 6: Effects of Parkin-mediated ubiquitination on VDAC1 oligomerization and cytosolic mtDNA accumulation in HepG2 cells.

From: Site-specific ubiquitination of VDAC1 restricts its oligomerization and mitochondrial DNA release in liver fibrosis

Fig. 6

a, b Relative ratio of cytosolic to total mtDNA (mt-Nd1 and mt-Nd6) determined by qPCR analysis of WT and ENDOG-knockdown HepG2 cells expressing Parkin induced by treatment with the selective mitochondrial division/mitophagy inhibitor Mdivi-1 (50 μM, 24 h) (n = 6/group). Representative immunoblots and quantitative histogram of (c) the results from a ubiquitination assay with WT Parkin and E3 activity-loss Parkin C431S (CS Parkin) (n = 6/group) and (d) VDAC1 oligomers (α-Tubulin as the loading control) (n = 6/group). The positions of VDAC1 monomers (Monomer), dimers (Di), trimers (Tri), and multimers (Multi) are indicated. e Parkin ubiquitinated VDAC1 with Lys27 ubiquitin linkages. WT or ENDOG-knockdown HepG2 cells expressed Myc-Parkin, HA-VDAC1, and Flag-Ub WT or K-only mutants (K6, K11, K27, K29, K33, K48, and K63) are indicated (n = 3/group). Mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001 and **** p < 0.0001 between the indicated groups.

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