Fig. 7: Identification of the VDAC1 ubiquitination site by Parkin. | Experimental & Molecular Medicine

Fig. 7: Identification of the VDAC1 ubiquitination site by Parkin.

From: Site-specific ubiquitination of VDAC1 restricts its oligomerization and mitochondrial DNA release in liver fibrosis

Fig. 7

a Protein sequences of the VDAC1 N-terminal domain in 5 species. b, c Three-dimensional human VDAC1 protein structure (Protein Data Bank, ID code 2JK4) showing the indicated ubiquitination sites. Representative immunoblots and quantitative histogram of (d) the results from a ubiquitination assay with VDAC1 WT and its mutants bearing Lys-to-Arg substitutions (N-KR, K274R, and K53R) (n = 6/group) and (e) VDAC1 oligomers (α-Tubulin as the loading control) (n = 6/group). f Ratio of cytosolic to total mtDNA in ENDOG-knockdown HepG2 cells expressing WT/CS Parkin and WT/K53R VDAC1 as indicated. Relative mRNA or protein levels of (g) IFNB1, (h) IL6, (i) p-STING/STING, and (j) p-IRF3/IRF3 in LX-2 cells incubated with conditioned media from ENDOG-knockdown HepG2 cells expressing WT/CS Parkin and WT/K53R VDAC1 as indicated. Mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001 and **** p < 0.0001 between the indicated groups.

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