Fig. 7: Intradiscal injection of lenti-Sirt5 or intraperitoneal injection of MB effectively alleviates compression-induced IDD in vivo. | Experimental & Molecular Medicine

Fig. 7: Intradiscal injection of lenti-Sirt5 or intraperitoneal injection of MB effectively alleviates compression-induced IDD in vivo.

From: SIRT5-related desuccinylation modification of AIFM1 protects against compression-induced intervertebral disc degeneration by regulating mitochondrial homeostasis

Fig. 7

RTC was performed in 12-week-old rats, followed by lenti-Sirt5 or MB treatment. a A schematic diagram of the operation. b Representative X-ray and MRI images of rat coccygeal vertebrae on Day 28 after surgery (n = 5). White arrows: points of injection; yellow triangles: uncompressed discs; red triangles: compressed discs; green triangles: compressed discs with lenti-Sirt5 or MB treatment. c Statistical graphs of Pfirrmann grades (n = 10). d Statistical graphs of DHI (n = 10). e Representative HE, SO, and TUNEL staining images. White scale bar: 100 μm; black scale bar: 500 μm. f Statistical graphs of histological scores (n = 5). g Statistical graphs of the percentage of TUNEL-positive cells (n = 5). h Representative images of immunofluorescence of SIRT5, Aggrecan, MMP13, and CHCHD4. Scale bar: 500 μm. Differences among multiple groups were analyzed by one-way ANOVA, while Pfirrmann grades and histological scores were analyzed by Kruskal‒Wallis h-tests. The data in the figures represent the mean ± S.D. The P value is shown, *P < 0.05, **P < 0.01.

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