Fig. 4: Retarded RGC axon growth in Vax1AA/AA mice.
From: Visuomotor anomalies in achiasmatic mice expressing a transfer-defective Vax1 mutant
a Schematic diagram depicting the 3D reconstitution of tdTom* fluorescent signals in RGC axons. Retinal cells, including RGCs, in Vax1+/+ and Vax1AA/AA littermate mouse embryos, which were cleared by the SHIELD method, were visualized by the tdTom* reporter expressed in the R26tm11 gene locus in a Pax6 α-Cre-dependent manner. The tdTom* signals of retinal cells and RGC axons, which grow in the OS to form the OC prior to extending through the OT, were then reconstituted in 3D (see details in Methods). b The 3D reconstituted images of E12.5, E13.5, and E14.5 mouse embryos are shown. c Relative tdTom* intensities in each domain of the RGC axon track, which is divided into 10 segments from the optic nerve head (OHD) to the vHT midline, are shown in the graphs. Cumulative tdTom* intensities, which represent the positions of RGC axon terminals, are also shown in the graphs. The values are averages (n = 3; 3 independent litters).
