Fig. 6: Ipsilaterally biased RGC axon projection in Vax1^AA/AA mice.

a Coronal sections of P30 mouse brains were stained with H&E. The sections containing the indicated commissural structures were identified and shown. AC, anterior commissure; CC, corpus callosum; D3V, dorsal third ventricle; FX, fornix; Pb, probus bundle; PC, posterior commissure; SC, superior colliculus; MTT, mammillo-thalamic tract. b Diagram depicting the fluorescence labeling of mouse RGCs. (c) Alexa 488- and Alexa 594-labeled CTB proteins were injected into the right and left eyes of the mice at P28, respectively. Brains of the CTB-injected mice were isolated at P30, and fluorescent signals emitted from the brains were visualized by an Axio Zoom stereoscope (Zeiss; three center columns). Then, the fluorescent signals in coronal sections of the brains were detected by an FV1000 confocal microscope (Olympus; rightmost column). Bright-field images of the brains were also taken to show the structure of the brains and optic nerves (leftmost column). CX, cerebral cortex; OB, olfactory bulb; *, ventral midline of HT equivalent to the position of the OC in Vax1^+/+ littermates. d Coronal sections of the CTB-injected mouse brains were collected, and fluorescent signals in the LGN area were detected. The areas surrounded by dotted lines are the dLGN. Contra, contralateral LGN section; Ipsi, ipsilateral LGN section. e The retinas and brains of P30 Vax1^+/+ and Vax1^AA/AA littermate mice carrying α-Cre;R26^tm4/+ transgenes, which express membrane-targeted EGFP (EGFP*) at the R26^tm4 gene locus upon Cre-dependent excision of the loxP-tdTomato*-loxP gene cassette, were isolated and visualized. The areas surrounded by dotted lines are SC. PT, pretectum.