Fig. 7: TRPV1 activation attenuated microglial phagocytosis of synapses in ApoE4 HFD mice. | Experimental & Molecular Medicine

Fig. 7: TRPV1 activation attenuated microglial phagocytosis of synapses in ApoE4 HFD mice.

From: TRPV1 regulates ApoE4-disrupted intracellular lipid homeostasis and decreases synaptic phagocytosis by microglia

Fig. 7

a, b Representative immunofluorescent images and quantification of PSD95 puncta in the cortex of ApoE3 and ApoE4 mice. c, d Representative immunofluorescence micrographs and quantification of PSD95 engulfed in Iba-1+ microglia (upper panel) and 3D reconstruction (lower panel) showing the volume of Iba-1 (green) and engulfed PSD95 puncta (red) in the cortex of ApoE3 and ApoE4 mice. e, f Representative immunofluorescent images and quantification of PSD95 (green) and CD68 (red) in the cortex of ApoE3 and ApoE4 mice. g Heatmap showing gene expression profiles enriched in the MHC-1 pathway of HFD-fed ApoE3 and ApoE4 mice. h, i Representative immunofluorescent images and quantification of NeuN+ and B2M+ cells in the cortex of ApoE3 and ApoE4 mice. n = 3 mice per group. Statistical tests: one-way ANOVA followed by Tukey’s post hoc test (b, d, f, i). Data represent the mean ± s.e.m. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Scale bars, 20 μm (a, c upper panel), 10 μm (c lower panel), 25 μm (f, h).

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