Fig. 4: Adenine base editing using cjABE with two tandem arrays of gRNAs via single AAV delivery.
From: Targeted dual base editing with Campylobacter jejuni Cas9 by single AAV-mediated delivery
A Schematic overviews of single-pAAV-cjABE and dual-pAAV-cjABE. The lengths between the two LTRs are shown. B Comparison of the base editing frequency of single-pAAV-cjABE containing a BGH polyA sequence or synthetic polyA sequences. C Comparison of the base editing frequency of single-pAAV-cjABE and dual-pAAV-cjABE with gRNAs containing synthetic polyA. D Base editing efficiency of dual-pAAV-cjABE at ANGPT2 and HPD-1 target sites following infection with AAV particles at a multiplicity of different viral genome (vg)/cell ranging from 1 × 102 to 1 × 105 vg/cell determined by quantitative PCR. E Analysis of potential off-target mutations in dual-pAAV-cjABE-treated cells. All experiments were conducted in biologically independent triplicates. Error bars indicate the standard error of the mean.
