Fig. 3: Fibulin2 expression in different cells deep within the bone.
From: Fibulin2: a negative regulator of BMSC osteogenic differentiation in infected bone fracture healing
a The differential expression of Fibulin2 in the BMSCs differentiated into osteocytes was detected by western blotting (WB). b The differential expression of Fibulin2 in the THP-1 cells differentiated into osteoclasts was detected by WB. The expression of Fibulin2 in BMSCs (c), osteoblasts (d and e), osteocytes (f), and osteoclasts (g) after stimulation with lipopolysaccharide (LPS) for 24 h was detected by WB. The expression of Fibulin2 in BMSCs (h), osteoblasts (i and j), osteocytes (k), and osteoclasts (l) after stimulation with LPS for 24 h was detected by ELISAs. Osteoblasts were derived from BMSCs after induction of osteogenic differentiation for 3 or 6 days; osteocytes were derived from BMSCs after osteogenic differentiation for 9 days; and osteoclasts were derived from THP-1 cells after osteoclast differentiation for 12 days.
