Fig. 3: CAR regulates HIF-1α activity in vivo and in vitro.
From: CAR links hypoxia signaling to improved survival after myocardial infarction
a Luciferase assay in HEK 293 cells cotransfected with HIF-1α Luc (a and b) or TA Luc reporter (c, d), RL-TK (internal control_Renilla Luciferase), and CAR Cherry or Cherry (n = 4). Twenty-four hours after transfection, the cells were either untreated or treated with 50 µM CoCl2 to induce chemical hypoxia (a, b) or with a gas mixture of 95% N2 and 5% CO2 to induce hypoxia for 10–12 min (c, d). Two-way ANOVA with Bonferroni’s post-test. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. e Cell viability after chemical hypoxia was reduced with higher concentrations of CoCl2 and overexpression of CAR versus the Cherry control. Two-way ANOVA with Bonferroni’s post-test. ***p < 0.001, **p < 0.01 and *p < 0.05 (n = 6).
