Fig. 5: CAR deficiency improves survival and contractile function after MI.
From: CAR links hypoxia signaling to improved survival after myocardial infarction
a Coronary artery ligation to induce MI. b Experimental design. Inducible heart-specific CAR KO mice at 7 weeks of age were injected with tamoxifen for two weeks. After one additional week, myocardial infarction (MI) was induced by ligation of the left anterior descending artery (LAD) at 10 weeks of age. Tissues were harvested after 0.25, 7 and 30 days. c CAR mRNA is upregulated in wild-type animals (WT) (light gray) but not in CAR-deficient animals (dark gray) after MI compared to the sham control. d Upregulation of CAR does not extend to the left ventricle (noninfarct zone). e Survival of CAR KO animals 7 days after MI was improved significantly compared to that of WT controls. f Sample traces of echocardiography M-mode with reduced mobility of the intraventricular septum (IVS) vs. unaffected left ventricular posterior wall (LVPW) in the WT after MI (flat line). Arrows indicate maximum contraction in systole. Quantification of echocardiography data is shown in Table 1. Data in b and c were analyzed for statistical significance with a two-way ANOVA, (n = 11). d was analyzed with a log-rank (Mantel‒Cox) test (n = 60).
