Fig. 2: Treatment with regorafenib resulted in attenuation of βPIX depletion-induced senescence in the lungs of mice. | Experimental & Molecular Medicine

Fig. 2: Treatment with regorafenib resulted in attenuation of βPIX depletion-induced senescence in the lungs of mice.

From: Defining regorafenib as a senomorphic drug: therapeutic potential in the age-related lung disease emphysema

Fig. 2

a Experimental scheme. SiRNAs were administered to mice by intratracheal injection. After 3 weeks, Reg (5 mg/kg) was administered orally once a day for 2 weeks. SA-β-Gal staining and immunohistochemistry (IHC) were performed on lung tissues. b Representative images of bronchioles and surrounding tissues. c ~ e Quantification of the data in b. Quantification of βPIX expression (c), SA-β-Gal staining (d), and p16INK4a expression (e). N = 5 mice per group. The error bars indicate the means ± SEMs, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, t-test. Scale bars, 50 μm.

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