Fig. 5: YTHDC1 inhibits the progression of bladder cancer partially through GLUT3.

a and b Reanalysis of RNA-seq data related to YTHDC1 and GLUT3 knockdown to identify the common target genes (a). KEGG enrichment analysis was performed with these common target genes (b). c and d T24 and 5637 cells were transfected with the indicated constructs for 48 h. The cells were harvested for western blot analysis (c) and a CCK-8 assay (d). The data are presented as the mean ± SEM of three replicates. ns not significant; ***P < 0.001. e–k T24 and 5637 cells were transfected with the indicated constructs for 48 h. The cells were harvested for western blot analysis (e) and CCK-8 (f), transwell (g), glucose consumption (h), lactate production (i), ECR (j) and OCR (k) assays. The data are presented as the mean ± SEM of three replicates. ns, not significant; ***P < 0.001. l–n T24 cells were transfected with the indicated constructs for 72 h. After puromycin selection, cells were collected and subcutaneously injected into nude mice. Images of the excised tumors are shown in Panel l. The tumor masses and tumor growth curves are shown in Panels m and n. The data are presented as the mean ± SEM of six replicates. ns not significant; ***P < 0.001.