Fig. 7: TRIM22 promotes the K63-linked ubiquitination of ERK1/2 through its RING domain. | Experimental & Molecular Medicine

Fig. 7: TRIM22 promotes the K63-linked ubiquitination of ERK1/2 through its RING domain.

From: TRIM22 promotes the proliferation of glioblastoma cells by activating MAPK signaling and accelerating the degradation of Raf-1

Fig. 7

a Anti-TRIM22 antibody was used to detect the binding of TRIM22 to the SPHK2/MAPK pathway core protein in U251MG, A172 and TJ905 cells. b Association of TRIM22 with ERK1/2 in U251MG, A172 and TJ905 cells. c, d Western blot analysis for ERK1/2 of Co-IPs with K63-linkage specific polyubiquitin antibody in the indicated modified U251MG, A172, and TJ905 cells. e Graphical model for TRIM22-mediated SPHK2/MAPK activation and GBM growth. Each experiment was repeated three times.

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