Table 1 GPCR ligands in CRHNs .
From: Olfactory modulation of stress-response neural circuits
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- Among the GPCRs expressed in CRHNs, those identified by three independent transcriptome analyses of single CRH + PVN neurons (Lee et al. [GSE143135; 18 cells], Xu et al. [GSE148568; 60 cells], and Romanov et al. [GSE74672; 86 cells]) are those for a variety of neuropeptides, neurotransmitters (glutamate, GABA, glycine, acetylcholine, adenosine, or ADP/ATP) and biogenic amines (epinephrine, norepinephrine, dopamine, histamine, and serotonin). Sequencing analyses of single neurons upstream of CRHNs (“Connect-seq”) revealed that all ligands (except melanin-concentrating hormone) were expressed in at least one of 117 hypothalamic neurons upstream of CRHNs. Pmch+ neurons upstream of CRHNs might be located in other brain regions. Although some receptors were expressed in only one cell out of a total of 126 CRH+ cells (red), all receptors (except GNRHR) for the ligands detected by Connect-seq were also found in at least one CRH+ cell. This table shows how applying sequencing analyses to both receptors in downstream neurons (e.g., CRHNs) and ligands in upstream neurons is a powerful approach for providing the genetic information necessary to dissect specific circuits at the molecular level.
