Fig. 6: Rnf146 overexpression increases excitatory synaptic transmission in prefrontal pyramidal neurons. | Experimental & Molecular Medicine

Fig. 6: Rnf146 overexpression increases excitatory synaptic transmission in prefrontal pyramidal neurons.

From: Dysregulation of the Wnt/β-catenin signaling pathway via Rnf146 upregulation in a VPA-induced mouse model of autism spectrum disorder

Fig. 6

a Spontaneous excitatory postsynaptic current (sEPSC) traces of prefrontal neurons in Rnf146- and eGFP-overexpressing mice. Scale bar: 50 pA, 500 ms. b The sEPSC amplitude of prefrontal neurons in Rnf146-overexpressing mice was significantly larger than that in eGFP-overexpressing mice (n = 13 cells from 5 mice for eGFP, n = 16 cells from 6 mice for Rnf146; two-tailed unpaired t test, t27 = 3.302, **P = 0.0027). c The sEPSC frequency of prefrontal neurons in Rnf146-overexpressing mice was significantly larger than that in eGFP-overexpressing mice (n = 13 cells from 5 mice for eGFP, n = 16 cells from 6 mice for Rnf146; two-tailed unpaired t test, t27 = 2.373, *P = 0.0250). d Spontaneous inhibitory postsynaptic current (sIPSC) traces of prefrontal neurons in Rnf146- and eGFP-overexpressing mice. Scale bar: 100 pA, 500 ms. e The sIPSC amplitude of prefrontal neurons in Rnf146-overexpressing mice is comparable to that in eGFP-overexpressing mice (n = 11 cells from 4 mice for eGFP, n = 12 cells from 4 mice for Rnf146; two-tailed unpaired t test, t21 = 1.980, P = 0.0609). f The sIPSC frequency of prefrontal neurons in Rnf146-overexpressing mice is comparable to that in eGFP-overexpressing mice (n = 11 cells from 4 mice for eGFP, n = 12 cells from 4 mice for Rnf146; two-tailed unpaired t test, t21 = 0.4323, P = 0.6699). g Representative traces of voltage responses of prefrontal neurons in Rnf146- or eGFP-overexpressing mice evoked by current step stimuli (−300-300 pA, 50 pA increment, 1 s). Scale bar: upper, 50 mV, 500 ms; lower, 500 pA, 500 ms. h Summary data of the number of action potentials evoked in response to 300 pA current steps. The number of AP spikes was comparable in the prefrontal neurons of Rnf146-overexpressing mice relative to those of eGFP-overexpressing mice (n = 13 cells from 5 mice for eGFP, n = 16 cells from 5 mice for Rnf146; two-way ANOVA with repeated measures: effect of injected current, F6, 162 = 28.24, ****P < 0.0001; effect of Rnf146 expression, F1, 27 = 0.006249, P = 0.9376; interaction between current injection and Rnf146 expression, F6, 162 = 0.7368, P = 0.6207, Sidak’s multiple comparison test for eGFP versus Rnf146. 50 pA, t189 = 0.1724, P > 0.9999; 100 pA, t189 = 0.6127, P = 0.9957; 150 pA, t189 = 0.7362, P = 0.9870; 200 pA, t189 = 0.3139, P > 0.9999; 250 pA, t189 = 0.1120, P > 0.9999; 300 pA, t189 = 0.5416, P = 0.9980; 350 pA, t189 = 0.7398, P = 0.9867).

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