Fig. 1: ATII cell-specific deletion of p300 prevents lung fibrosis. | Experimental & Molecular Medicine

Fig. 1: ATII cell-specific deletion of p300 prevents lung fibrosis.

From: The deubiquitinase UCHL3 mediates p300-dependent chemokine signaling in alveolar type II cells to promote pulmonary fibrosis

Fig. 1

a Representative images showing p300 IHC in lung samples from patients with IPF and control subjects. Scale bars, 100 µm. The dot plot represents the p300 intensity/hematoxylin ratio of patients with IPF (n = 42) and control subjects (n = 16). Control: 0.7293 (0.0844–2.4565) vs. IPF: 2.536 (0.6019–12.2473). b Immunofluorescence staining of p300 was performed on IPF lungs. The intensity of p300 was calculated using ZEN 3.0 software. n = 4 per group. Two-tailed t test with Welch’s correction. c The intensity of p300, CBP, PCAF, TIP60, and GCN5 protein expression was calculated using ImageJ. Control subjects (n = 6) and IPF (n = 11-17). d Quantitative analysis of P300 IHC and collagen in lung samples in mice with BLM-induced pulmonary fibrosis. The dot plot represents the p300 intensity/hematoxylin ratio or blue region of MTS. n = 5–11 mice/group. e Immunofluorescence analysis of human lungs using the indicated antibodies. The cell markers are as follows: Pro-SPC, ATII cells; CCSP, club cells; FoxJ1, ciliated cells; PDPN, ATI cells; Muc5AC, goblet cells; and α-SMA, fibroblasts. The weighted colocalization coefficient was calculated using ZEN 3.0 software. n = 3 per group. f, i, l, o Representative MTS-stained lung sections from control and cell-specific p300 KO mice ((f), Spc-p300f/f ATII cell-specific KO; (i) Ccsp-p300f/f club cell-specific KO; (l) Foxj1-p300f/f ciliated cell specific; (o) Spc-p300f/f in Ccsp-TGF-β1-TG mice with or without tamoxifen treatment). Scale bar, 50 µm. g, j, m, p The deposition of collagen (blue) was quantified in MTS-stained lung samples from (g) Spc-p300f/f, (j) Ccsp-p300f/f, (m) Foxj1-p300f/f, and (p) Ccsp-TGF-β1-TG mice using ImageJ. h, k, n, q Collagen levels in lung samples from (h) Spc-p300f/f, (k) Ccsp-p300f/f, (n) Foxj1-p300f/f, and (q) Spc-p300f/f in Ccsp-TGF-β1-TG mice were assessed using the Sircol collagen assay. n = 3–15 mice/group. All average data are the mean ± s.e.m. n.s. not significant; *P < 0.05, **P < 0.01, ****P < 0.0001, Statistical analysis was performed using a two-tailed Mann–Whitney U test (a, c–e) or ANOVA with Tukey’s test (g–q).

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