Fig. 4: ATII cell–specific deletion of p300 inhibits macrophage polarization. | Experimental & Molecular Medicine

Fig. 4: ATII cell–specific deletion of p300 inhibits macrophage polarization.

From: The deubiquitinase UCHL3 mediates p300-dependent chemokine signaling in alveolar type II cells to promote pulmonary fibrosis

Fig. 4

a Flow cytometry showed the percentages of M2 (CD45+F4/80+CD206+) macrophages in the tissues of Spc-p300f/f or Spc-p300d/d mice. SSC, side scatter. b, c Relative expression of Arg1, Cd206, Cd163, Cox-2, and Cxcl10 in mouse lung samples. n = 6. d, e Immunofluorescence analysis of CD206 (green) and F4/80 (red) with DAPI (blue) in the lungs. Arrowheads indicate colocalized cells. Scale bar = 50 μm. f Flow cytometric analysis of the expression of the M2 marker CD206 in MH-S murine alveolar macrophages cultured with or without CM from control or p300 knockdown RLE-6TN cells. g qRT‒PCR analysis of the M2 markers Arg1, Cd206, and CD163 in MH-S cells cultured with or without CM from control or p300 knockout RLE-6TN cells. Statistical analysis was performed with ANOVA with Tukey’s test. Error bars represent the mean ± s.e.m. ns not significant, **P < 0.01, ***P < 0.001, and ****P < 0.0001.

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