Fig. 6: Lpc-EV treatment reduced microgliosis in the brains of Tg-APP/PS1 mice. | Experimental & Molecular Medicine

Fig. 6: Lpc-EV treatment reduced microgliosis in the brains of Tg-APP/PS1 mice.

From: Lactobacillus-derived extracellular vesicles counteract Aβ42-induced abnormal transcriptional changes through the upregulation of MeCP2 and Sirt1 and improve Aβ pathology in Tg-APP/PS1 mice

Fig. 6

a–d Photomicrographs showing the anti-Iba-1-stained parietal cortex (Pacx), dorsal hippocampus (dHP), and piriform cortex (Piricx) of wild-type mice (WT), Tg-APP/PS1 mice (Tg), and Tg-APP/PS1 mice treated with Lpc-EV (Tg+Lpc-EV) (a). Higher magnification of the boxed areas in the parietal cortex of the indicated groups (b). Relative ratio of total anti-Iba-1-stained fluorescent intensity (c) and total area of anti-Iba-1-stained cells (d) in the parietal cortex, dorsal hippocampus, and piriform cortex of the indicated groups. S1, somatosensory cortex 1; BLA, basolateral nucleus of amygdala. n = 8 animals for WT and Tg and 11 for Tg+Lpc-EV. Total intensity; F(2,23) = 16.17, p < 0.0001; F(2,23) = 13.85, p = 0.0001; F(2,23) = 19.43, p < 00.0001. Total anti-Iba-1-stained area; pacx, F(2,23) = 20.66, p < 00.0001; hp, F(2,23) = 14.78, p < 00.0001; piricx, F(2,23) = 28.27, p < 00.0001. e–g Photomicrographs (e) showing Iba-1-stained areas of microglia (red) surrounding ThS-stained plaques (green) in the parietal cortex of Tg-APP/PS1 mice (Tg) and Tg-APP/PS1 mice treated with Lpc-EV (Tg+Lpc-EV). Relative ratio of Iba-1-stained areas (f) and the intensity of Iba-1-stained microglia (g) over plaque areas. n = 8–9 animals. Iba-1-stained area ratio; t(15) = 0.7881, p = 0.4429; Iba-1-stained intensity ratio; t(15) = 1.677, p = 0.1143. Data are presented as mean ± SEM. *p < 0.05; **p < 0.01 (Student’s t-test; and one-way ANOVA followed by the Newman‒Keuls post hoc test).

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