Fig. 5: Generation of transparent mouse tibia samples by passive bone clearing methods.
From: Optimization of the optical transparency of bones by PACT-based passive tissue clearing
a Schematic representation and timeline of the passive bone clearing methods. The individual reagents or processes used in the fourteen clearing methods for the decalcification and clearing process are shown. b Comparison of optical transparency achieved in mouse tibia samples processed using Bone-mPACT+ (with 20% EDTA), Bone-CLARITY, CUBIC-L/R, MACS, Ce3D, Fast 3D Clear, EZ Clear, PEGASOS, BABB, methanol BABB, 3DISCO, uDISCO, FDISCO, and BoneClear. The transparency of all the cleared samples was evident when they were placed on a patterned background (length × width = 5 × 5 mm).
