Fig. 4: Ang 1-7 promoted osteoclast differentiation in mouse cells. | Experimental & Molecular Medicine

Fig. 4: Ang 1-7 promoted osteoclast differentiation in mouse cells.

From: Angiotensin receptor blockers, but not angiotensin-converting enzyme inhibitors, inhibit abnormal bone changes in spondyloarthritis

Fig. 4

a Level of Ang 1-7 measured using ELISAs in osteoclast culture media with/without ACEi (1 × 10−5 M captopril). b, c Mouse bone marrow monocytes were treated with Ang II (1 × 10−6 M) or Ang 1-7 (1 × 10−6 M) with/without ARB (1 × 10−5 M losartan). b Representative TRAP staining images (left) and the number of TRAP-positive MNCs (>3 nuclei) per well (right). c RT‒qPCR analysis. d Western blotting analysis of Ang II- or Ang 1-7-treated RAW 264.7 cells. Hash indicates nonspecific bands. e–g Representative TRAP staining images (left) and the number of TRAP-positive MNCs (>3 nuclei) per well (right) before and after Ang II (1 × 10−6 M) administration with/without ACE2i (1 × 10−5 M MLN-4760) (e), Ang I (1 × 10−6 M) with/without NEPi (1 × 10−5 M sacubitrilat) (f), and Ang I with/without MasRi (1 × 10−5 M A779) (g). Scale bar = 50 µm. ACE2i, angiotensin-converting enzyme 2 inhibitor; Ang, angiotensin; MasRi, Mas receptor inhibitor; MNCs, multinuclear cells; NEPi, neprilysin inhibitor; TRAP, tartrate-resistant acid phosphatase. Values are presented as the mean ± S.D.; *p < 0.05, **p < 0.01 using the Mann‒Whitney U test.

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