Fig. 8: cFAM210A inhibits the phosphorylation of YBX1, suppressing its transactivation function toward MET.
a Western blot results showing the protein levels of MET, YBX1 and P-YBX1S102 upon overexpression or silencing of cFAM210A. b qRT‒PCR results showing the expression of CD44, MET and MDR1 upon overexpression or silencing of cFAM210A. c The correlation between the RNA level of MET and that of cFAM210A in 80 ANL tissues was analyzed. d Western blot analysis following immunoprecipitation (IP) after overexpression or silencing of cFAM210A. e–g qRT‒PCR (e), Western blot (f) and MET promoter luciferase reporter assay (g) results showing that OSU-03012 suppressed the phosphorylation of YBX1S102 and downregulated the expression of MET, while these effects were abolished by silencing cFAM210A. h–i The CCK-8 assay (OD450 was measured on the third day) (h) and in vitro limiting dilution assay (i) results demonstrated that MET promoted the proliferation and stemness of HCC cells, while these effects were abolished by overexpressing cFAM210A. For (b), (e), (g) and (h), Student’s t test was used. For (i), the estimated stem cell frequency of each group was calculated by ELDA (http://bioinf.wehi.edu.au/software/elda/). NC negative control; cFAM210A-oe cFAM210A-overexpressing lentivirus, cFAM210A-sh lentivirus-mediated short hairpin RNA against cFAM210A, ns not significant. *P < 0.05; **P < 0.01; ***P < 0.001.
