Fig. 1: nCas9 in the base editor generated unintended indels at the target sites.
From: Precise base editing without unintended indels in human cells and mouse primary myoblasts
a–d Comparisons of C-to-T or A-to-G substitutions and indel frequencies by BE3 and AncBE4max or ABE and ABEmax at each of four human target sequences in HEK293T cells. e Top three indel patterns in the HEK3 target sequence induced by nCas9-based CBEs. f Top three indel patterns in the HBB-E2 target sequence induced by nCas9-based ABEs. Red arrowheads and dotted lines, cleavage sites induced by SpCas9; blue letter, PAM sequence; underline, target sequences. Control indicates the untreated group. Each dot represents an individual target experiment. The data are shown as the mean ± S.D. of three independent experiments.
